Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Computer controlled expansion of equine cord blood mesenchymal stromal cells on microcarriers in 3 L vertical-wheel ® bioreactors

doi: 10.3389/fbioe.2023.1250077

Figure Lengend Snippet: Antibodies used for flow cytometry.

Article Snippet: CD44 , Horse , Bio-Rad , FITC , CVS18 , , MSC.

Techniques: Cytometry

Journal: Pathology and Oncology Research

Article Title: Overexpression of CD44v8-10 in Colon Polyps—A Possible Key to Early Diagnosis

doi: 10.3389/pore.2021.614281

Figure Lengend Snippet: Structure of human CD44 protein (A) . The blue line represents alternative region that is completely missing in CD44s isoform. Variable region of CD44 is a site of heavy O-glycosylation. The globular amino terminal domain of CD44 contains three disulfide bonds and two hyaluronan binding motifs . (B) Representative endoscopic images of colon adenomas we sampled. The top image displays serrated adenoma while the bottom image displays conventional adenoma. The images are taken in two modes, i.e., white light and narrow band image (NBI).

Article Snippet: The antibodies used were anti-actin IgG (AC-15, Sigma), anti-pan CD44 IgG (2C5 BBA10, R&D Systems), anti-CD44v10 IgG (AB2082, Millipore), anti-mouse IgG-HRP (A4416, Sigma), anti-rabbit IgG-HRP (NA934VS, GE Healthcare).

Techniques: Glycoproteomics, Binding Assay

Journal: Pathology and Oncology Research

Article Title: Overexpression of CD44v8-10 in Colon Polyps—A Possible Key to Early Diagnosis

doi: 10.3389/pore.2021.614281

Figure Lengend Snippet: Clinical significance of various CD44 isoforms in certain cancer types. A reduced list of CD44 prognostic markers in various cancer types. met., metastasis; neg., negative.

Article Snippet: The antibodies used were anti-actin IgG (AC-15, Sigma), anti-pan CD44 IgG (2C5 BBA10, R&D Systems), anti-CD44v10 IgG (AB2082, Millipore), anti-mouse IgG-HRP (A4416, Sigma), anti-rabbit IgG-HRP (NA934VS, GE Healthcare).

Techniques:

Journal: Pathology and Oncology Research

Article Title: Overexpression of CD44v8-10 in Colon Polyps—A Possible Key to Early Diagnosis

doi: 10.3389/pore.2021.614281

Figure Lengend Snippet: Overexpression of CD44v8-10 in colon polyps (A,B) Position of the selected primers in CD44 mRNA exon structure. Constant exons (always transcribed) are marked orange. Variable exons are marked blue. (A) 8v8 primers were used for detection of inclusion of variable exons v8-10, i.e., CD44v8-10. Product size of 398 bp confirms transcription of variable exons v8-10. (B) CD44s1 primers span the whole variable region of the CD44 gene. Product size of 368bp relates to the standard isoform of CD44 (CD44s). Product size >368 bp indicates inclusion of variable exons. (C,D) Agarose gel resolution of end-point PCR products of 8v8 primers (C) and CD44s1 primers (D) in polyp and healthy tissue samples. Actin was used as a loading control. PCR products for actin are displayed below. The arrows indicate product representing CD44v8-10 (C) and CD44s (D) . Relative quantification using densitometric analysis of agarose gels is represented by the bar graph (E) . An agarose gel after resolution of the PCR products of CD44v3 primers that start at exon v3 and finish at the constant region (F) . Keratinocytes were used as a positive control for exon inclusion while fibroblast express mostly CD44s. Increasing size of PCR products means higher number of variable exons included in the final mRNA transcript. Yellow dashed line delineates grouping of two gels into one picture. Samples from patients with sessile serrated adenoma (SSA) are marked with asterisk (*); samples of villous adenomas are marked (V); samples of tubular adenomas are marked (T); samples of tubulovillous adenomas are marked (TV).

Article Snippet: The antibodies used were anti-actin IgG (AC-15, Sigma), anti-pan CD44 IgG (2C5 BBA10, R&D Systems), anti-CD44v10 IgG (AB2082, Millipore), anti-mouse IgG-HRP (A4416, Sigma), anti-rabbit IgG-HRP (NA934VS, GE Healthcare).

Techniques: Over Expression, Agarose Gel Electrophoresis, Control, Quantitative Proteomics, Positive Control

Journal: Pathology and Oncology Research

Article Title: Overexpression of CD44v8-10 in Colon Polyps—A Possible Key to Early Diagnosis

doi: 10.3389/pore.2021.614281

Figure Lengend Snippet: CD44v8-10 overexpression in colon polyps. Position of real time primers within the CD44 mRNA transcript of CD44v8-10 (A) and CD44s (B) . Reverse primers span exon junctions and anneal only if the following exon junctions occur C5+V8 (A) or C5+C15 (B) . Verification of appropriate size of PCR products was performed in random samples using 3% agarose gels (C) . Relative expression levels of CD44s and CD44v8-10 in colon polyps compared to adjacent healthy mucosa (D) . Statistical values are calculated using one sample t test. Column mean is significantly different from hypothetical value 1.0; p = 0.018 for v8-10; p < 0.001 for CD44s number of patients N = 50.

Article Snippet: The antibodies used were anti-actin IgG (AC-15, Sigma), anti-pan CD44 IgG (2C5 BBA10, R&D Systems), anti-CD44v10 IgG (AB2082, Millipore), anti-mouse IgG-HRP (A4416, Sigma), anti-rabbit IgG-HRP (NA934VS, GE Healthcare).

Techniques: Over Expression, Expressing

Journal: Pathology and Oncology Research

Article Title: Overexpression of CD44v8-10 in Colon Polyps—A Possible Key to Early Diagnosis

doi: 10.3389/pore.2021.614281

Figure Lengend Snippet: Expression of CD44 receptor in the mucosa of adenomatous polyp. Biopsy specimens of colon polyp were frozen sectioned and immunolabeled using pan specific anti-CD44 and anti-CD44v10 IgG. Nuclei were stained with DAPI (Blue). Nonspecific binding of secondary FITC IgG was checked (upper right panel). The data indicate that the most of CD44 protein is expressed in the epithelial cells of the colon crypt base.

Article Snippet: The antibodies used were anti-actin IgG (AC-15, Sigma), anti-pan CD44 IgG (2C5 BBA10, R&D Systems), anti-CD44v10 IgG (AB2082, Millipore), anti-mouse IgG-HRP (A4416, Sigma), anti-rabbit IgG-HRP (NA934VS, GE Healthcare).

Techniques: Expressing, Immunolabeling, Staining, Binding Assay

Journal: Pathology and Oncology Research

Article Title: Overexpression of CD44v8-10 in Colon Polyps—A Possible Key to Early Diagnosis

doi: 10.3389/pore.2021.614281

Figure Lengend Snippet: mRNA levels of some known splicing regulators. SR-like proteins and non SR-like ESRPs were tested in the cohort of 50 patients using real time PCR. ESRP-1 was almost two-fold overexpressed in colon polyps in 50 patients (A) . Expression level of ESRP-2 was similar in both types of colon mucosa. Relative expression levels of SR-like proteins in colon polyps compared to normal mucosa (B) . Relative expression levels of ESRP-1 and ESRP-2 in three patients whose polyps had the lowest rate of alternative splicing (C) . A graphical overview of suggested mechanism running in the background of ASP of CD44 in colon polyps (D) . The yellow background color highlights the site responsible for aberrant alternative splicing. The scheme was taken from our previous publication .

Article Snippet: The antibodies used were anti-actin IgG (AC-15, Sigma), anti-pan CD44 IgG (2C5 BBA10, R&D Systems), anti-CD44v10 IgG (AB2082, Millipore), anti-mouse IgG-HRP (A4416, Sigma), anti-rabbit IgG-HRP (NA934VS, GE Healthcare).

Techniques: Real-time Polymerase Chain Reaction, Expressing, Alternative Splicing

Journal: Pathology and Oncology Research

Article Title: Overexpression of CD44v8-10 in Colon Polyps—A Possible Key to Early Diagnosis

doi: 10.3389/pore.2021.614281

Figure Lengend Snippet: HA-FITC binds to CD44 receptor. The upper right panel of immunoblots shows CD44s protein expression in H1299, T47D cells and cells extracted from adenomatous (32p) and healthy colon (32) mucosa using anti-CD44 IgG and anti-actin IgG as a loading control. The part of the verification western blot was also published in Ref. . The histograms show fluorescence intensity of cells incubated with FITC labelled hyaluronan, natural ligand of CD44s receptor. CD44 non-expressing T47D cells were used as a negative control (black line); H1299 cells were used as a positive control for CD44-HA interaction (blue line); Cells extracted from the colon polyp (red line); cells extracted from healthy colon mucosa (green line). The histogram images are representatives of three independent experiments.

Article Snippet: The antibodies used were anti-actin IgG (AC-15, Sigma), anti-pan CD44 IgG (2C5 BBA10, R&D Systems), anti-CD44v10 IgG (AB2082, Millipore), anti-mouse IgG-HRP (A4416, Sigma), anti-rabbit IgG-HRP (NA934VS, GE Healthcare).

Techniques: Western Blot, Expressing, Control, Fluorescence, Incubation, Negative Control, Positive Control

Journal: iScience

Article Title: Interleukin 11 confers resistance to dextran sulfate sodium-induced colitis in mice

doi: 10.1016/j.isci.2023.105934

Figure Lengend Snippet:

Article Snippet: PE Anti-Human/Mouse CD44 (IM7) Antibody , TONBO biosciences , 50-0441; RRID:AB_2621762.

Techniques: Purification, Polymer, Plasmid Preparation, Recombinant, In Situ, Software

Journal: International Journal of Cancer

Article Title: Co-expression of osteopontin and CD44v9 in gastric cancer

doi: 10.1002/(SICI)1097-0215(19980417)79:2%3C127::AID-IJC5%3E3.0.CO;2-V

Figure Lengend Snippet: FIGURE 3 – In situ hybridization for OPN mRNA in human gastric carcinomas (a–f) and immunohistochemistry of OPN (g, h) and CD44v9 (i, j) in human stomach cancers and their lymph node metastases. Positive reactions are denoted by red-color reaction product. a: well differentiated stomach cancer (case 537). c and d: tumor cells that had massive invasion into muscularis propria (case 93-4502) and lymph node metastasis. Intense staining for OPN mRNA was observed in the cytoplasm of cancer cells. b: negative control. e and f: primary tumor (case 309) and lymph node metastasis (case 93-5390). Inflammatory cells in tumor stroma showed strong positive signals for OPN mRNA (arrow heads). g and i: intralymphatic vascular component in poorly differentiated stomach cancer. Tumor cells exhibit cluster formation in lymphatic vessel and showed intense staining for both OPN and CD44. h and j: lymph node metastasis of poorly differentiated type stomach cancer (case 93-5390). Co-immunoreaction of OPN and CD44v9 was retained in metastatic focus. Scale bar, 50 µm.

Article Snippet: The sections were treated consecutively at room temperature with anti-CD44v9 mAb (diluted 1:2000) or anti-OPN antibody (diluted 1:200) for 3 hr, biotinylated anti-rabbit or mouse IgG horse serum (diluted 1:100, Vector, Burlingame, CA) for 30 min, and avidin DH-biotinylated horseradish peroxidase complex (Vectastain ABC kit, Vector) for 30 min. Peroxidase staining was performed for 10–15 min using a solution of 3,38-diaminobenzidine tetrahydrochloride in 50 mM Tris-HCl (pH 7.5) containing 0.001% hydrogen peroxide.

Techniques: In Situ Hybridization, Immunohistochemistry, Staining, Negative Control

Journal: BMC Veterinary Research

Article Title: Osteochondral regeneration of the femoral medial condyle by using a scaffold-free 3D construct of synovial membrane-derived mesenchymal stem cells in horses

doi: 10.1186/s12917-021-03126-y

Figure Lengend Snippet: Percentages (%) of cells positive for specific molecular markers by flow cytometry

Article Snippet: After incubation, the antibodies against CD11a/CD18, CD44, MHC class I, and MHC class II were coupled with secondary got antibodies conjugated to fluorescein isothiocyanate (FITC; FITC anti-mouse IgG, Rockland lmmunologicals).

Techniques:

Journal: Toxicologic pathology

Article Title: Differentiation patterns of uterine carcinomas and precursor lesions induced by neonatal estrogen exposure in mice

doi: 10.1177/0192623318779326

Figure Lengend Snippet: Antibodies used for immunohistochemistry

Article Snippet: Rabbit anti-rat (CD44) , , Vector Laboratories , BA-4001 , Y0809 , – , 1 μg/mL , –.

Techniques: Concentration Assay, Plasmid Preparation, Negative Control

Journal: Toxicologic pathology

Article Title: Differentiation patterns of uterine carcinomas and precursor lesions induced by neonatal estrogen exposure in mice

doi: 10.1177/0192623318779326

Figure Lengend Snippet: A, CD44 expression in the basal and suprabasal layers of stratified squamous epithelium in the ectocervix and scattered basal cells (arrowhead) of the transition zone. B and C, CD44 expression in scattered glands. D, CD44 expression in neoplastic glands. Slides for all images were immunostained for CD44 (A-D). Original objective 20× (A), 20× (B), 60× (C), and 60× (D).

Article Snippet: Rabbit anti-rat (CD44) , , Vector Laboratories , BA-4001 , Y0809 , – , 1 μg/mL , –.

Techniques: Expressing

Journal: Toxicologic pathology

Article Title: Differentiation patterns of uterine carcinomas and precursor lesions induced by neonatal estrogen exposure in mice

doi: 10.1177/0192623318779326

Figure Lengend Snippet: Immunohistochemical expression of differentiation markers in the uterine epithelium following neonatal exposure to DES or GEN

Article Snippet: Rabbit anti-rat (CD44) , , Vector Laboratories , BA-4001 , Y0809 , – , 1 μg/mL , –.

Techniques: Immunohistochemical staining, Expressing, Marker, Labeling, TUNEL Assay